1. Services for Submitted Samples
In addition to our open-access membership service, CMSF offers several options for analyzing individual samples. Our rates are per-sample, regardless of the type of analysis requested (effective May 1, 2012):
University of California Community: $45
Other Academic/Non-Profit Institutions: $62
If this is your first time submitting samples to our facility, we recommend that you contact us prior to submitting your samples so that we may best address your specific scientific needs.
For UCD clients, our sample submission form can be downloaded here: ms_samplesub_form_1.pdf. Don't forget to include your recharge number, e-mail address, and molecular formula and structure (if known). Please bring your completed form along with your clearly-labeled samples to 9 Hutchison Hall during normal business hours.
UCD Clients; please contact us first for any work you may wish done that is under contract with an outside business.
Off-campus clients need to contact us first regarding their samples and analysis needs. We have a fillable PDF Customer Information Form as well as a pre-approved Delegated Agreement (basic legal contract) which must be signed and returned before any samples are submitted for amounts >$500. If your company or university makes any substantial changes to the pre-approved Delegated Agreement, those changes must first be reviewed by our Contracts Office. Additionally, you may wish to check with your administrative office how they wish to be billed as well before submitting samples.
The Campus Mass Spectrometry Facilities are supported by a number of funding sources. Please acknowledge the facility in your publications by stating "Analysis for this project was performed in the UC Davis Campus Mass Spectrometry Facilities."
Electrospray Ionization Services:
The following services utilize the electrospray ionzation technique to ionize your sample. Samples must be free of salts and detergents, and soluble in water or a volatile solvent such as methanol or acetonitrile. Polar solvent systems such as DMSO or DMF are not permitted. If your sample requires a buffer, volatile buffers such as ammonium acetate can be used in place of sulfate, phosphate, or tris buffer systems.
Our LTQ-Orbitrap instrument is capable of high resolution accurate mass measurements. This small molecule is measured within 0.0005 Da of its theoretical mass.
High Resolution Mass Spectrometry (HRMS)
This service utilizes our state of the art high resolution (up to 100,000 FHWM) Orbitrap mass spectrometer to obtain an accurate mass measurement (< 5 ppm) of your compound of interest. This service is typically used for small molecules, peptides, oligonucleotides, and some intact proteins (up to approx. 30 kDa). The analyte must be within the mass range of 50-4000 m/z as well as capable of forming an ion in solution.
Liquid Chromatography Mass Spectrometry (LCMS)
This service also utilizes our Orbitrap mass spectrometer, coupled to a high performance liquid chromatography system, for the separation of a mixture of analytes before ionization and mass analysis as well as an Agilent triple-quadrupole mass spectrometer. This service is generally utilized for crude extracts and tryptic digests of proteins. Additionally, this service is available on a microfluidic system coupled to our new Xevo G2 Qtof mass spectrometer on a per-sample basis.
LC-MS & Static Nanospray Ionization (nanoESI) Time of Flight Molecular Weight
This service utilizes a Time of Flight (TOF) instrument equipped with either a C4 chip based LC column or a static nanospray source for the ionization of small amounts of sample. This instrument offers mass accuracies of about 10 ppm and can analyze samples of up to 150 kDa. This service is typically most useful when HRMS analysis is not feasible, and when the experiment demands a higher degree of mass accuracy than can be provided by the MALDI instrument. A typical sample best-suited for this service is an intact protein over 30 kDa.
Matrix Assisted Laser Desorption Ionization (MALDI) Services:
The following services utilize the MALDI technique to ionize your sample. Samples that are salt-free give the best data, but if necessary, MALDI can tolerate salts and buffers up to ~50 mM. Polar solvent systems such as DMSO or DMF are not permitted.
(MALDI) Molecular Weight
This service uses a Nd:YAG laser for desorption and ionization of your sample from a stainless steel plate, and is typically used for peptides, peptide mass fingerprinting, oligonucleotides, polymers, and especially intact proteins. The Time of Flight (TOF) mass analyzer on our MALDI instrument offers resolutions approaching 40,000 FHWM for analytes between 500-10,000 m/z, and mass accuracies typically below 10ppm. This instrument can operate in the lower-resolution linear mode for the analysis of proteins and polymers up to 500 kDa.
2. Membership Information
Our facility is pleased to offer open-access use of mass spectrometry instrumentation to members of the UC Davis community. Our annual memberships include consultation and training on one instrument for up to two users, access to the facilities, and technical support. Our current rate is $1652 per membership (effective July 1, 2012), is billed on the fiscal year basis, and current members can operate an additional instrument (eg, MALDI and ESI) for $500/yr. Our new triple-quad LCMS service is $1652/yr by itself (can't be added as a 2nd instrument). We only have a limited number of LCMS slots/year and samples are typically run in the PM into the evening.
Please contact us for more information.